Review



egfp  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc egfp
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/bio_rxiv__64898__2025__11__30__691375-33-15-16?v=Addgene+inc
    Average 94 stars, based on 121 article reviews
    egfp - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "N-acetyl-L-leucine (Levacetylleucine) normalizes Transcription Factor EB (TFEB) activity by stereospecific bidirectional modulation"

    Article Title: N-acetyl-L-leucine (Levacetylleucine) normalizes Transcription Factor EB (TFEB) activity by stereospecific bidirectional modulation

    Journal: bioRxiv

    doi: 10.64898/2025.11.30.691375

    HeLa cells were transfected with TFEB-EGFP (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Figure Legend Snippet: HeLa cells were transfected with TFEB-EGFP (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).

    Techniques Used: Transfection, Incubation, Staining, Translocation Assay, Concentration Assay, Derivative Assay, Control

    TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of either NALL (black) or L-Leucine (LL, green). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 104-161 cells, and significance determined by nonparametric ANOVA test (A) or unpaired t-test (B), with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to the vehicle control, i.e. 0 mM. Comparing NALL and LL: #, p <0.05; ###, p < 0.001.
    Figure Legend Snippet: TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of either NALL (black) or L-Leucine (LL, green). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 104-161 cells, and significance determined by nonparametric ANOVA test (A) or unpaired t-test (B), with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to the vehicle control, i.e. 0 mM. Comparing NALL and LL: #, p <0.05; ###, p < 0.001.

    Techniques Used: Translocation Assay, Incubation, Control

    TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of each enantiomer: the L-form (NALL, black), D-form (NADL, green) or the racemic mixture of both (orange). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 153-265 cells, and significance determined by nonparametric ANOVA test, with significance denoted by * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to vehicle control, i.e. 0 mM. Comparing NALL and NADL or racemic mixture, using a nonparametric ANOVA test: ##, p < 0.01; ###, p < 0.001.
    Figure Legend Snippet: TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of each enantiomer: the L-form (NALL, black), D-form (NADL, green) or the racemic mixture of both (orange). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 153-265 cells, and significance determined by nonparametric ANOVA test, with significance denoted by * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to vehicle control, i.e. 0 mM. Comparing NALL and NADL or racemic mixture, using a nonparametric ANOVA test: ##, p < 0.01; ###, p < 0.001.

    Techniques Used: Translocation Assay, Incubation, Control



    Similar Products

    egfp  (Addgene inc)
    94
    Addgene inc egfp
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/bio_rxiv__64898__2025__11__30__691375-33-15-16?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    egfp - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    pegfp n1 mitf m  (Addgene inc)
    94
    Addgene inc pegfp n1 mitf m
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Pegfp N1 Mitf M, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/pmc12618667-480-110-111?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    pegfp n1 mitf m - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    pegfp n1 tfeb  (Addgene inc)
    93
    Addgene inc pegfp n1 tfeb
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Pegfp N1 Tfeb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/pmc12618667-480-117-118?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    pegfp n1 tfeb - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier
    gfp ni tfeb plasmid  (Addgene inc)
    94
    Addgene inc gfp ni tfeb plasmid
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Gfp Ni Tfeb Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/bio_rxiv__2025__09__11__675705-301-10-12?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    gfp ni tfeb plasmid - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    tfeb gfp plasmid  (Addgene inc)
    94
    Addgene inc tfeb gfp plasmid
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Tfeb Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/pmc12276339-52-22-24?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    tfeb gfp plasmid - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    control cells  (Addgene inc)
    94
    Addgene inc control cells
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Control Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/pmc12276339-52-18-24?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    control cells - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    p egfp n1 tfeb  (Addgene inc)
    94
    Addgene inc p egfp n1 tfeb
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    P Egfp N1 Tfeb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/pmc12276339-179-23-24?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    p egfp n1 tfeb - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier
    plasmid expressing tfeb gfp  (Addgene inc)
    94
    Addgene inc plasmid expressing tfeb gfp
    HeLa cells <t>were</t> <t>transfected</t> with <t>TFEB-EGFP</t> (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).
    Plasmid Expressing Tfeb Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+tfeb/pmc12276339-162-9-13?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    plasmid expressing tfeb gfp - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    HeLa cells were transfected with TFEB-EGFP (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).

    Journal: bioRxiv

    Article Title: N-acetyl-L-leucine (Levacetylleucine) normalizes Transcription Factor EB (TFEB) activity by stereospecific bidirectional modulation

    doi: 10.64898/2025.11.30.691375

    Figure Lengend Snippet: HeLa cells were transfected with TFEB-EGFP (A-C) or TFEB-mScarlet3 (D,E) and then incubated with NALL at different concentrations or times. Cells were then counter-stained with the NucSpot 488 (D,E) or NucSpot 650 (A-C) to label the nucleus and TFEB translocation quantified by colocalization with NucSpot. (A) Single-cell images of TFEB-EGFP (green) and NucSpot 650 (magenta). (B,C) Concentration-response of HeLa treated for 18 hrs with NALL. Nuclear localization is plotted as either the Pearson’s coefficient (B) or derived as the percentage of cells with nuclear TFEB (C). (D,E) Time course of the effect of 2 mM NALL upon TFEB, either expressed as the Pearson’s coefficient (D) or the percentage of cells with nuclear TFEB (E). The dotted lines (B,D) highlight a correlation coefficient of zero used for thresholding the percentage of nuclear cells. Data are expressed as the mean ± SEM of 179-215 cells, and significance determined by ANOVA test, with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to respective control, 0 mM (B,C) or t = 0 (D,E).

    Article Snippet: Per well, cells were transfected with 100 ng TFEB tagged on its C-terminus with either EGFP (Addgene plasmid # 38119) or mScarlet3 (produced in-house) for 4-6 hrs.

    Techniques: Transfection, Incubation, Staining, Translocation Assay, Concentration Assay, Derivative Assay, Control

    TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of either NALL (black) or L-Leucine (LL, green). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 104-161 cells, and significance determined by nonparametric ANOVA test (A) or unpaired t-test (B), with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to the vehicle control, i.e. 0 mM. Comparing NALL and LL: #, p <0.05; ###, p < 0.001.

    Journal: bioRxiv

    Article Title: N-acetyl-L-leucine (Levacetylleucine) normalizes Transcription Factor EB (TFEB) activity by stereospecific bidirectional modulation

    doi: 10.64898/2025.11.30.691375

    Figure Lengend Snippet: TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of either NALL (black) or L-Leucine (LL, green). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 104-161 cells, and significance determined by nonparametric ANOVA test (A) or unpaired t-test (B), with significance depicted as * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to the vehicle control, i.e. 0 mM. Comparing NALL and LL: #, p <0.05; ###, p < 0.001.

    Article Snippet: Per well, cells were transfected with 100 ng TFEB tagged on its C-terminus with either EGFP (Addgene plasmid # 38119) or mScarlet3 (produced in-house) for 4-6 hrs.

    Techniques: Translocation Assay, Incubation, Control

    TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of each enantiomer: the L-form (NALL, black), D-form (NADL, green) or the racemic mixture of both (orange). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 153-265 cells, and significance determined by nonparametric ANOVA test, with significance denoted by * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to vehicle control, i.e. 0 mM. Comparing NALL and NADL or racemic mixture, using a nonparametric ANOVA test: ##, p < 0.01; ###, p < 0.001.

    Journal: bioRxiv

    Article Title: N-acetyl-L-leucine (Levacetylleucine) normalizes Transcription Factor EB (TFEB) activity by stereospecific bidirectional modulation

    doi: 10.64898/2025.11.30.691375

    Figure Lengend Snippet: TFEB-EGFP translocation in HeLa cells incubated for 18 hrs with different concentrations of each enantiomer: the L-form (NALL, black), D-form (NADL, green) or the racemic mixture of both (orange). Translocation is expressed either as the Pearson’s correlation coefficient with NucSpot 650 (A) or as the percentage of cells with nuclear TFEB (B). Data are expressed as the mean ± SEM of 153-265 cells, and significance determined by nonparametric ANOVA test, with significance denoted by * p < 0.05, ** p < 0.01, or *** p < 0.001 compared to vehicle control, i.e. 0 mM. Comparing NALL and NADL or racemic mixture, using a nonparametric ANOVA test: ##, p < 0.01; ###, p < 0.001.

    Article Snippet: Per well, cells were transfected with 100 ng TFEB tagged on its C-terminus with either EGFP (Addgene plasmid # 38119) or mScarlet3 (produced in-house) for 4-6 hrs.

    Techniques: Translocation Assay, Incubation, Control